HepatoSure™ 100-donor Pooled Human Hepatocytes

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PackagingCharacterization Provided

Now with Expanded Characterization Data!

HepatoSure™ now provides the most comprehensive characterization data on the market. Each lot is characterized for activity of numerous CYP and non-CYP enzymes, including multiple cytochrome P450s, UDP-Glucuronosyltransferases (UGT), Sulfotransferases (SULT) and aldehyde oxidase (AO). We also characterize for several hepatic uptake transporters, including OATP1B1 (estrone-3-sulfate), OATP1B3 (CCK-8), OCT1 (MPP+) and NTCP (taurocholate). Finally, each lot is provided with intrinsic clearance data.

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Order HepatoSure™ online at the XenoTech eStore.

HepatoSure™ is XenoTech’s 100-donor pool of cryopreserved human hepatocytes. The largest pool on the market, HepatoSure™ provides the best value when it comes to consistency of data reproducibility and the ability to produce assured minimum yields (AMY). HepatoSure™ pushes the envelope to deliver higher quality, more consistency and a better overall value than any other pooled hepatocyte product currently available.

Pooled human hepatocytes are a preferred test system in many drug discovery and development applications which require intact cellular systems for in vitro testing. Intact hepatocytes contain the major hepatic drug-metabolizing enzymes and co-factors required to evaluate the metabolism and potential drug-drug interactions of drug candidates effectively.

HepatoSure™ Optimization Kit

XenoTech’s HepatoSure™ pooled human cryopreserved hepatocytes are shipped with a HepatoSure™ Optimization Kit. This kit contains a nutrient rich media to ensure consistent yield and high viability post-thaw. Each kit is sufficient to thaw and isolate up to three 1.5mL vials of HepatoSure™. When using the optimization kit in conjunction with XenoTech’s HepatoSure™ pooled cryopreserved human hepatocyte product, you are guaranteed a post-thaw viability of 70% or higher.

HepatoSure™ Comparison

HepatoSure™ was tested against a comparable product currently on the market and demonstrated similar viability, enzymatic activity and overall performance. Costing less than the competition, HepatoSure™ includes 100 donors, provides the necessary media for thawing and isolation (HepatoSure™ Optimization Kit) and delivers high quality, consistent hepatocytes.

Download the HepatoSure™ product flyer

Features

• 100 donors (reduces interindividual variability)
• Most extensive characterization on the market
• High viability and enzyme activity levels
• Maintains viability >50% up to 4 hours
• Fully characterized LC/MS/MS methods
• Best overall value on the market
• Study Manager, Study Director and QC review of data

Packaging

New 100-Donor Pool

• 1.5 mL (Catalog #HCP100.H15)
• AMY: 5 million cells (Assured Minimum Yield)
• HepatoSure™ Optimization Kit included (Catalog #K2600)

Characterization Provided

• % Viability
• CYP1A2 Phenacetin O-dealkylation
• CYP2A6 Coumarin 7-hydroxylation
• CYP2B6 Bupropion hydroxylation
• CYP2C8 Amodiaquine N-dealkylation
• CYP2C9 Diclofenac 4´-hydroxylation
• CYP2C19 S-Mephenytoin 4´-hydroxylation
• CYP2D6 Dextromethorphan O-demethylation
• CYP2E1 Chlorzoxazone 6-hydroxylation
• CYP3A4/5 Testosterone 6β-hydroxylation
• CYP3A4/5 Midazolam 1´-hydroxylation
• UGT 7-Hydroxycoumarin glucuronidation
• SULT 7-Hydroxycoumarin sulfonation

Transporter

• OCT1 (1-Methyl-4-phenylpyridinium iodide)
• OATP1B1 (Estrone-3-sulfate)
• OATP1B3 (CCK-8)
• NTCP (Taurocholate)

Intrinsic Clearance Data

Aldehyde Oxidase Activity

Products

Product #	Name	Gender	Amount	Assured Minimum Yield
HCP100.H15	HepatoSure cryo human hepatocytes, 100 donors, mixed gender	Mixed	1.5 mL	5 million cells