Beagle Dog Liver Subcellular Fractions

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PackagingCharacterization Provided

Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S-trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.

Packaging

Micro­somes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA. 

Characterization Provided

The following lot-specific product information is included with each order of non-human liver microsomes.

• Specific content of cytochrome P450
• Specific content of cytochrome b5
• NADPH-cytochrome c reductase activity
• 7-Ethoxycoumarin O-dealkylation
• Animal Information and Husbandry

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