Cynomolgus Monkey Intestine Subcellular Fractions

View Products

PackagingCharacterization ProvidedSafety, Handling, and StorageRelated Products

Enterocyte metabolic enzymes perform a significant role in the first-pass metabolism of many orally ingested xenobiotics.

The use of adequately active and stable intestine subcellular fractions is highly recommended in determining a more complete analysis of first-pass metabolism of ingested xenobiotics.  Consequences of intestinal biotransformation include toxification by bioactivation and detoxification by aiding in excretion. The majority of metabolic enzymes, including cytochrome P450 enzymes and UGT enzymes, are contained in the mature enterocytes of the villus tips. The submucosa, muscularis and serosa yield low microsome quantities and are not used in the preparation of XenoTech's intestinal subcellular fractions. 

Our unique processing method allows for optimal P450 expression, which yields activities higher than most commercially available intestinal subcellular fractions. Microsomes should be thawed in a room temperature water bath and unused portions returned to an ultra-low freezer (–70°C or colder) as soon as possible.

Packaging

Micro­somes
Concentration: 10 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 4 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl, EDTA, glycerol, heparin, PMSF, leupeptin, dithiothreitol, and aprotinin

Characterization Provided

All intestine subcellular fractions are characterized for the following activities:

• NADPH-cytochrome c reductase
• Testosterone 6β-hydroxylation
• 4-Methylumbelliferone glucuronidation

Safety, Handling, and Storage

Policy Statement Concerning the Safety of Non-human Primate-derived Material

XenoTech manufactures products derived from non-human primate tissue for the purpose of xenobiotic research. All tissue used to manufacture these products is obtained from reliable sources within the United States, and is obtained from disease-free (asymptomatic) animals under the care of a veterinarian.

In accordance with federal (USDA) regulations, all non-human primates entering the United States are placed in quarantine for a period of thirty days. If an animal is infected with Ebola virus, it would die during the quarantine period, and hence, would not reside in a primate colony in the United States. For this reason, Ebola virus testing is not a common procedure in the United States. Animals are tested for Simian Herpes B virus (SBV), Simian Retrovirus (SRV), and Simian Immunodeficiency virus (SIV). All non-human primate tissue utilized at XenoTech is obtained from animals which have tested seronegative for these viruses.

Although animals are quarantined and tested for certain infectious diseases, it is not scientifically possible to guarantee that non-human primate-derived material is free of all infectious agents and poses absolutely no health risk. Therefore, we strongly caution all researchers who use non-human primate-derived material to treat such material as a potential biohazard, and to observe all national, regional, and local regulations governing the handling of such material. Even if non-human primate-derived material has been tested for the presence of specific infectious agents by highly sensitive techniques, such as RT-PCR, and found to test negative, the material should still be treated as a potential biohazard.

Please note:  XenoTech complies with all regulations promulgated by the Convention on International Trade in Endangered Species (CITES). Accordingly, special permits and arrangements must be made to ship all primate products internationally.  Customers in Europe and Japan should contact our distributor nearest you for information on pricing and availability.

Related Products

XenoTech also offers subcellular fractions of intestinal tissue from the following toxicologically significant species:

• Cynomolgus Monkey
• Beagle dog
• IGS Sprague-Dawley rat

For other extrahepatic fractions, contact us and ask about our Custom Products option.  We have experience preparing a variety of unique research products for a growing list of customers.

Note: Intestinal subcellular fractions are prepared with a cocktail of protease inhibitors including PMSF (Phenylmethylsulfonylflouride)  to protect the P450 enzymes and are not recommended for esterase assays.

Products