Cynomolgus Monkey Kidney Subcellular Fractions

PackagingCharacterization ProvidedSafety, Handling, and StorageRelated Products

Drug-metabolizing enzymes in the kidney play a significant role in the metabolism of many xenobiotics. Kidneys have many metabolic responsibilities including the hydroxylation of fatty acids and their derivatives, which is one of the functions of the CYP4A and CYP4B enzymes expressed in renal microsomes. There is increasing interest in renal metabolism due to the significant role that kidneys play in the metabolism of drugs and the fact that the kidney is a major site of drug-induced toxicity.

To assess the stability of human and animal renal subcellular fractions, pooled samples were subjected to several freeze-thaw cycles, and analyzed for lauric acid 12-hydroxylation, umbelliferone glucuronidation and NADPH-cytochrome c reduction.  As shown below, renal samples prepared by XenoTech’s subcellular fractionation protocol can be repeatedly frozen and thawed at least ten times without significant loss of CYP4A activity.  Similar results were obtained for umbelliferone glucuronidation and NADPH-cytochrome c reduction.

Packaging

Microsomes
Concentration:  10 mg/mL
Suspension buffer:  250 mM sucrose

S9 fraction
Concentration:  5 mg/mL
Suspension buffer:  50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

• NADPH-cytochrome c reductase
• Lauric Acid 12-hydroxylation
• 4-Methylumbelliferone glucuronidation

Safety, Handling, and Storage

Policy Statement Concerning the Safety of Non-human Primate-derived Material

XenoTech manufactures products derived from non-human primate tissue for the purpose of xenobiotic research. All tissue used to manufacture these products is obtained from reliable sources within the United States, and is obtained from disease-free (asymptomatic) animals under the care of a veterinarian.

In accordance with federal (USDA) regulations, all non-human primates entering the United States are placed in quarantine for a period of thirty days. If an animal is infected with Ebola virus, it would die during the quarantine period, and hence, would not reside in a primate colony in the United States. For this reason, Ebola virus testing is not a common procedure in the United States. Animals are tested for Simian Herpes B virus (SBV), Simian Retrovirus (SRV), and Simian Immunodeficiency virus (SIV). All non-human primate tissue utilized at XenoTech is obtained from animals which have tested seronegative for these viruses.

Although animals are quarantined and tested for certain infectious diseases, it is not scientifically possible to guarantee that non-human primate-derived material is free of all infectious agents and poses absolutely no health risk. Therefore, we strongly caution all researchers who use non-human primate-derived material to treat such material as a potential biohazard, and to observe all national, regional, and local regulations governing the handling of such material. Even if non-human primate-derived material has been tested for the presence of specific infectious agents by highly sensitive techniques, such as RT-PCR, and found to test negative, the material should still be treated as a potential biohazard.

Please note:  XenoTech complies with all regulations promulgated by the Convention on International Trade in Endangered Species (CITES). Accordingly, special permits and arrangements must be made to ship all primate products internationally.  Customers in Europe and Japan should contact our distributor nearest you for information on pricing and availability.

Related Products

XenoTech also offers subcellular fractions of intestinal tissue from the following toxicologically significant species:

• Cynomolgus Monkey
• Beagle dog
• IGS Sprague-Dawley rat

For other extrahepatic fractions, contact us and ask about our Custom Products option.  We have experience preparing a variety of unique research products (from human and laboratory animal resources) for a growing list of customers.