New Zealand Rabbit Subcellular Fractions

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PackagingCharacterization ProvidedProduct Details

View microsome lot information and characterization data here!

View S9 lot information and characterization data here!

Liver microsomes con­tain cytochromes P450, flavin-monooxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glu­cur­ono­syl­transferases and other drug-metabolizing enzymes, and are intended for use in in vitro studies of xenobiotic metabolism.

The S9 fraction is a mixture of microsomes and cytosol. Accordingly, it contains a wide variety of Phase 1 and Phase 2 enzymes including P450 enzymes, flavin-mono­oxygenases, carboxyl­esterases, epoxide hydrolase, UDP-glucuronosyl­transferases, sulfotransferases, methyl­transferases, acetyl­trans­ferases, gluta­thione S-trans­fer­ases and other drug-metabolizing enzymes.

Liver cytosol contains many xenobiotic metabolizing enzymes including carboxylesterases, soluble epoxide hydrolases, alcohol dehydrogenases, aldehydes oxidases, xanthine oxidases, diamine oxidases, sulfotransferases, glutathione S-transferases, etc.

Packaging

Micro­somes
Concentration: 20 mg/mL
Suspension buffer: 250 mM sucrose

S9 fraction
Concentration: 20 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Cytosol
Concentration: 10 mg/mL
Suspension buffer: 50 mM TRIS·HCl (pH 7.4 at 4°C) containing 150 mM KCl and 2 mM EDTA

Characterization Provided

The following lot-specific product information is included with each order of non-human liver microsomes.

•  Specific content of cytochrome P450
•  Specific content of cytochrome b5
•  NADPH-cytochrome c reductase activity
•  7-Ethoxycoumarin O-dealkylation
•  Animal Information and Husbandry

Product Details

NOMENCLATURE Crl:KBL(NZW)BR ORIGIN The NZW rabbit was obtained in 1991 by Charles River Canada (CRC) from Kitayama Labs K.K. of Nagano Prefecture, Japan. The colony had been rederived by Kitayama Labs in 1985. COAT COLOR Albino.

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