IGS Sprague-Dawley Rat Skin Subcellular Fractions

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Many drug metabolizing enzymes of the liver and other visceral organs are also expressed in the skin. The skin possesses a broad spectrum of xenobiotic metabolizing enzymes including cytochromes P450, flavin monooxygenases, esterases/amides, UDP-glucuronosyltransferases, glutathione S-transferases and sulfotransferases. Specifc activities of these cutaneous enzymes are typically less than 10% of those found in the liver, yet may play a significant role in the biotransformation of xenobiotics absorbed and/or delivered through the skin.

Microsomes and S9 fractions are prepared from dorsal full-thickness rat skin tissue and are intended for in vitro research only.

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Microsomes

Concentration: 10mg/mL
Volume: 0.25mL
Suspension buffer: 250mM sucrose

S9 Fraction

Concentration: 5mg/mL
Volume: 1.0mL
Suspension buffer: 50mM TRIS HCl (pH 7.4 at 4°C) containing 150mM KCl and 2 mM EDTA

Characterization Provided

The following lot-specific product information is included with each order of skin microsomes and S9:

• NADPH-cytochrome c reductase activity
• Testosterone 6β-hydroxylation
• 4-Methylumbelliferone glucuronidation
• Clopidogrel hydrolysis
• Methylprednisolone 21-hemisuccinate hydrolysis
• Animal Information and Husbandry

Product Details

Animal Model Details

NOMENCLATURE Crl:CD (SD)IGS BR ORIGIN Originated in 1925 by Robert W. Dawley from a hybrid hooded male and female Wistar rat. To CRL in 1950 from Sprague Dawley, Inc. Caesarean rederived in 1955 from original Charles River Sprague Dawley® colonies. In 1981, 8 colonies were selected to form the IGS Foundation Colony. Rederived into isolator foundation colony in 1997. IGS refers to animals bred using the CRL International Genetic Standard system. COAT COLOR Albino

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